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KMID : 0895820120220020031
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Journal of Oriental Rehabilitation Medicine
2012 Volume.22 No. 2 p.31 ~ p.45
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Effect of Fermented Black Rice Extract on NH4Cl and Acetaminophen-induced Hepatotoxicity
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Park Jong-Min
Park Sun-Ok
Shambhunath Bose
Lee Su-Jin
Jeon Song-Hee
Song Mi-Young
Kim Ho-Jun
Lee Myeong-Jong
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Abstract
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Objectives :This study was carried out to investigate the hepato-protective effects of fermented black rice(Oryza sativa Linne) extracts(FBRE) through of HepG2 cell and rats.
Methods :In vitro, the cell viability, ammonia level, liver enzymes including AST, ALT, GST and SOD were investigated after the treatment of fermented black rice extract(FBRE) and positive controls of commercial nutraceutical products in NH4Cl-treated HepG2 cell lines. In vivo, liver function biomarkers were measured in acetaminophen hepatotoxicity animal model. 32 male rats were divided into four groups and each group were fed different formula for 4 days, i.e, normal control(C), acetaminophen treatment(AA), Hepakhan 250 mg/kg(commercial product as positive control) + acetaminophen treatment(HK+AA), fermented black rice extracts 250 mg/kg + acetaminophen treatment(FBRE+AA). Weight, food intake, liver enzymes, lipid-protein profiles were investigated.
Results :In vitro, FBRE and two commercial products(Hepaglucan and Helpkhan) had shown to improve the cell viability, ammonia level, GPT, SOD in HepG2 cell and there were, however, no change on GST, GOT observed. FBRE had shown that similar or better hepato-protective effects than other commercial products. In vivo experiment, there were no significant change in food intake but body weight decreased in all acetaminophen-treatment groups(p<0.05). GPT and glucose level was significantly decreased in FBRE+AA group(p<0.05).
Conclusions :These results suggest that FBRE has potential possibility to improve compromised hepatic function.
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KEYWORD
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hepato-protective effects, fermented black rice, Oryza sativa Linne extracts, HepG2 cell, rats Liver cell
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